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Group 1: six-spined shortfin gobies 1
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Group 2: six-spined shortfin gobies 2
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Group 3: seven-spined shortfin gobies
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Group 4: longfin gobies
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Group 5: divided pelvic-fin gobies
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allied family Ptereleotridae
allied family Eleotridae
allied family Microdesmidae
Introduction to the gobies
The gobies are the largest family of reef fishes and account for a major fraction of the world's tropical marine fish fauna. There are well over a hundred Caribbean species, and doubtless a few more to be described. In addition, there are numerous cryptic species among the gobies in the western Atlantic (populations with sharply divergent DNA sequences that are usually allopatric, but can be sympatric). Although almost always small and inconspicuous, gobies occur in large numbers in all reef-associated habitats. There are over 30 regional genera and, unfortunately, many groupings of closely related species that make species-level larval identifications particularly challenging. I have managed to identify and include in this guide the larvae of almost all of the shallow-water goby genera of the region; a few deep-water genera remain unknown.
The great taxonomic diversity of gobies is certainly reflected in their early life history stages. Larval gobies exhibit the full range of larval sizes at transition, from about 4 mm to almost 30 mm SL. In general, however, they are small and nondescript with a long, narrow, and thin body. They tend to have small to medium-sized terminal mouths, small heads without spines, and slender flexible spines in the fins. They can be recognized most readily by their two separated dorsal fins with the first having only a few spindly spines. In addition, they often have fused pelvic fins and typically light markings. The basic marking pattern for goby larvae is a ventral midline series of melanophores: at the isthmus, pelvic-fin base, anal-fin base, and caudal peduncle, along with a variety of other small melanophores. Some larval gobies also have markedly narrowed and tilted eyes. Since Caribbean goby genera are often quite speciose and the larvae only become distinct during transition or even later, some groups will certainly require DNA testing for the identification of individual larvae to the species level.
How to divide the gobies up?

Since there are too many Caribbean species to deal with on one webpage, the goby family needs to be subdivided, a task that has tested more than a few fish taxonomists and can be quite frustrating. A variety of divisions have been proposed in the past, none of which have been satisfactory and certainly none have been backed up by strong phylogenetic evidence. Gobies are highly variable in morphology and genetics and deep phylogenies so far are quite elusive. Suffice to say, some traditional separations based on the state of fusion of the pelvic fins and the presence or absence of pores and scales are not reflective of true relationships. Indeed, the state of the pelvic fins can be variable among larvae of obviously close relatives. The evolutionary loss of pores and scales is an individual adaptation and not likely a shared attribute among relatives. Larval markings in gobies are often sparse and the basic patterns are generally shared by unrelated groupings and do not fall out in manageable blocks.

Since genetic relatedness is an unwieldy method to subdivide larval forms in a group this complex, I have tried to arrange the groups in a form that makes it easier to navigate. The basic separations I use are six vs. seven first-dorsal-fin spines, the short and long-fin groups, i.e. the increasing number of median-fin rays, and the fusion state of the pelvic fins.

The number of dorsal-fin spines, six vs. seven, is not always easy to see on larvae, but is consistent enough to be useful and seems a natural separation among gobies. The number of dorsal and anal-fin soft rays is somewhat consistent within similar-appearing larvae and the "long-fin" gobies with more than 11 second-dorsal and anal-fin elements are usually easy to distinguish from the "short-fin" gobies, typically with 9, 10, or 11 second-dorsal and anal-fin elements. Lastly, although pelvic-fin states can be phylogenetically labile, the state of fusion is often an obvious visible attribute of goby larvae and the division of the pelvic fins seems to be a characteristic of a set of goby species as well as the allied gobioids, the eleotrids and ptereleotrids.

Group 1:
Short-fin gobies 1 (six-spined)


Bathygobius, Lophogobius, Priolepis, Sicydium, Awaous

Group 2:
Short-fin gobies 2 (six-spined)


Coryphopterus and Lythrypnus

Group 3:
Short-fin gobies (seven-spined)


Barbulifer, Elacatinus, Tigrigobius, Gobiosoma, Risor, Ginsburgellus

Group 4:
Long-fin gobies


Evermannichthys, Ctenogobius, Gnatholepis, Nes, Evorthodus, Ctenogobius, Bollmannia, Gobionellus, Microgobius, Palatogobius

Group 5:
Divided pelvic-fin gobies


Psilotris, Pycnomma, Gobulus, Chriolepis

GOBIES OF THE CARIBBEAN

there are a total of about 120 Caribbean species

Sources for taxonomy and fin-ray counts include Fishbase, Gobiidae.com, the FAO key, Randall's Caribbean Reef Fishes, Peterson Field Guides Atlantic Coast Fishes, Bohlke and Chaplin's Fishes of the Bahamas, McEachran and Fechhelm's Fishes of the Gulf of Mexico, Richards' Early Stages of Atlantic Fishes goby chapter, Bohlke and Robins' Western Atlantic Gobies (PANS Phila.) and their Revision of ... Coryphopterus and specific literature.
 
   
Quick Key to Genera: in order of increasing anal-fin elements  
 
 
Pelvic fins anal-fin elements 1st dorsal spines anal fewer than dorsal equal anal more than dorsal
Psilotris divided 7-11 7 x
Varicus divided 8 7 x x
Chriolepis divided 7-12 7 x x
Pycnomma divided 9 7 x
Gobulus partial 10-11 7 x
Robinsichthys divided 11 7 x
FAMILY ELEOTRIDAE divided 9-10 6 x x x
Bathygobius fused 9 6 x
Lythrypnus fused 9 6 x
Lophogobius fused 9 6 x
Priolepis fused 9 6 x
Coryphopterus 1 fused 9-11 6 x
Coryphopterus 2 divided 9-11 6 x x
Barbulifer fused 9 7 x
Gobiosoma fused* 9-10 7 x
Tigrigobius fused 9-10 7 x
Elacatinus fused 10-12 7 x
Ginsburgellus fused 10 7 x
Risor fused 10 7 x
Evermannichthys fused 9-12 3-7 x
Awaous fused 10 6 x
Sicydium fused 11 6 x
Gnatholepis fused 12 6 x
Nes fused 11-13 7 x
Evorthodus fused 12 6 x
Ctenogobius fused 12-13 6 x
Oxyurichthys fused 14 6 x
Vomerogobius fused 13 6 x
Bollmannia fused 12-14 7 x
Parrella fused 13 7 x x
Gobionellus fused 15 6 x
Akko fused 15 7 x
Gobioides fused 16-17 7 x
Microgobius fused 16-21 7 x x
Palatogobius fused 21 7 x
Pariah 8
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Larval Gobies
Goby larvae are typically the most abundant larvae collected in most reef fish larval collections, both in diversity and often in total numbers. Indeed, Ctenogobius saepepallens, the dash goby, is the most frequently occurring larval type in my Panama collections, followed closely by the bridled gobies, Coryphopterus spp.
Since the process of elimination is critical to the identification of larval gobies, the diversity within this group makes for some difficulty in species ID. A variety of other factors add to the complexity of identifying goby larvae:
  • Melanophore Patterns
  • fin-ray Counts
  • Transformation
  • Size Variation
  • Larval Eye Morphology
  • pelvic-fin Morphology
    Melanophore patterns
    The larval melanophore patterns within the family tend to be conservative, with many larval types sharing a sparse basic pattern of a ventral midline series of melanophores: at the isthmus, pelvic-fin base, anal-fin base, and caudal peduncle. Melanophore patterns can be quite variable within types- many individuals, especially earlier-stage larvae, are missing one or a few of the standard complement for their type.
     
    Melanophores can be contracted, appearing as discrete dots, or expanded into either complex dendritic star-shapes or linear forms. Linear melanophores often merge with adjacent melanophores into long streaks. In addition, the intensity of melanophores can vary a great deal, with many preserved larvae showing faint or indistinct melanophore patterns (in some species this variation is
    pronounced, for example in larval Ctenogobius). Furthermore, melanophores on the delicate membranes between the fin rays can easily be lost in handling as the fins get frayed and thus the frequency of these markings in the few larval types that have these are undoubtedly higher than observed. Fin membrane melanophores occur on several common larval gobies, including Bathygobius soporator, Coryphopterus glaucofraenum and Microgobius signatus, as well as on some eleotrids. (photographs of melanophore streaks in larval Eleotris amblyopsis right and contracted vs. expanded in Bathygobius mystacium below) larval eleotris
    larval bathygobius larval bathygobius
    fin-ray counts
    The soft fin-ray counts often vary by at least one or two in Caribbean gobies, unlike many other reef fish families that have very conservative fin-ray formulas. In addition, the reported modal fin-ray counts from different sources in the literature can sometimes vary, usually by one ray. Nevertheless, modal fin-ray counts are critical to species diagnosis. larval psilotris
     
    The oft-used character of six vs. seven spines in the first dorsal fin is sometimes difficult to see (since the seventh spine is tiny). Although it is often not that useful for practical screening of larvae, the number of spines can be very useful for genus diagnosis, separating genera with otherwise similar appearances and fin-ray counts. There is some variation in dorsal spine counts; but it is helpful to recognize that six-spined gobies usually have five close spines and then a distant sixth, while seven-spined gobies have five close spines and then two more spaced out farther. Thus some of the variants can be recognized as anomalous (i.e. four close and two spaced out is likely a variant seven-spined goby).
    A common problem is that some literature sources count total dorsal-fin spines and total dorsal-fin soft-rays, confusing whether the spine count is including the first, often spinous, element of the second soft dorsal fin. It is best to count total elements in the second-dorsal and anal fins to avoid this problem (and the issue of whether the first element of the second-dorsal fin and anal fin in some gobies is spinous or soft, which... surprise, can also vary).
    Transformation
     
    Larval gobies tend to initiate transformation from larval to juvenile phase (also transition, or metamorphosis) while still pelagic and many transitional individuals can be collected in waters over the reef. Indeed, in some collections, the majority of specimens are in transition. As a result, larval goby samples can often include a surprisingly wide range of morphological appearances.
     
    Head Shape: The head shape of transitional gobies varies greatly. Pre-transformation goby larvae usually have thin pointed heads with terminal mouths. As they initiate transformation, the head usually thickens and the snout often becomes more rounded. In those species with blunt head-profiles, this change can be marked and the mouth can move subterminally. (photographs below of larval Gobionellus oceanicus transitional series)
    larval goby fish larval gobiidae fish larval gobionellus
     
    Eye Shape: The eye shape can change radically during transition and the process is somewhat consistent within larval types. Both the shape itself and the size at which the changes are observed can be an important character for species identification. As larvae initiate transformation, narrowed eyes become round, tilted eyes become vertical, and in some species the eye becomes markedly larger (in a few it gets smaller, e.g. larval Nes longus). Eye shapes can thus be valuable for inferring the stage of goby larvae, i.e. in a species with narrowed eyes in pre-transitional larvae, the presence of round eyes in a small individual indicates that it is in transition. This becomes particularly useful in practical larval sorting where the size at which larvae develop round eyes can be an important character, as in Lythrypnus vs. Coryphopterus. (photograph at right of transitional changes in the eyes of larval Lythrypnus nesiotes) larval lythrypnus
     
    Body Shape: The body of pre-transitional larvae is typically thin and becomes thicker and bulkier at transition. This change needs to be distinguished from effects of condition of larvae. Clearly some emaciated larvae appear very thin and narrow. This appearance can be found in some larvae with round eyes and even metamorphic melanophores, indicating that they are not just immature early-stage larvae.
     
    The fins of those species who develop long pectoral and pelvic fins as juveniles show a marked increase in the length of these fins at transition. In a few cases, where juveniles have a characteristically short fin, that fin length may decrease at transformation.
     
    There is variation in the timing of changes in the early life history of gobies; some larval gobies develop transitional morphological changes, especially rounded eyes and blunted snouts, before acquiring any transitional markings, as in the larval Lythrypnus at right. In contrast, it is common with larval gobies to see individuals of the same species and in the same collection that have started to develop metamorphic melanophores while still morphologically in mid-transition, at least in body and head shape. However, the eyes of larval gobies almost always start rounding before transitional markings develop; it is exceptionally rare to see a larva with dense metamorphic melanophore patches and narrow eyes. Two larvae at the ends of the spectrum easily look like they could be different species. larval fishes
    fish larvae
    Metamorphic Melanophores: These arrays of additional melanophores (along with leukophores and iridophores) are usually smaller and limited to the skin surface, compared to the large, discrete, and often deeply-penetrating larval melanophores. In many other reef fish families, the metamorphic melanophores are typically in dense patches that often begin on the head and develop posteriorly following the pattern of the juvenile markings of the species. In gobies, however, the size difference of the melanophores is less obvious, and metamorphic melanophores can often be just as large as larval melanophores and are distinguished mostly by their graded appearance, i.e. the accumulation of more markings in a pattern starting around the mouth and head, then at the caudal peduncle and dorsal midline, and then filling in from forward to rear (photographs below of a transitional series of Bathygobius soporator). This phenomenon helps a great deal in providing missing links for species IDs, but also contributes to the confusing variety in the appearance of larval types. This is especially the case when the metamorphic melanophores can show up in very different sequences, as is common in larval Coryphopterus glaucofraenum.
    larval coral reef fish
    larval bathygobius soporator
    larval goby
    Size Variation
     
    Of course there is some variation in the size of larvae within a species. There can be two sources of this variation and distinguishing between them is important.
     
    One is the simple size increase with growth and development during the early life history: younger and less-developed larvae are smaller than older ready-to-settle larvae. This variation can be detected by the well-known ontogenetic landmarks to be expected with growth, i.e. first the flexion of the notochord, then the full development of the fin-ray elements and finally the eye and head shape changes as settlement approaches. Among the late-stage larvae collected over reefs, almost all have passed the flexion stage and have developed their full complement of fin rays. The subsequent body and eye-shape changes and the degree of development of metamorphic melanophores are the features that vary most in these settlement-stage larvae.
    The second source of variation is individual variation in size at the same stage of development. This variation can be large in gobies, and, of course, the observed range increases with sample size. This variation can be confusing, and the occasional extreme size variant can look like a different species entirely. For example, the photograph at right shows the extreme one percent variation in size at transformation for the common Coryphopterus glaucofraenum larval type. Note that these are all transitional larvae that have already developed round eyes. The larval sizes in the photograph range from 5.1 to 8.6 mm SL, but 90% of the larvae of this species that I have collected are concentrated between 6.5 and 7.3 mm SL. larval coryphopterus
    Larval eye morphology
     
    Larval gobies of different species and different stages of development exhibit a remarkable variety of shapes of the eyeball, most often a narrow vertical oval but, in some species, irregular or even squared. These eye shapes, along with other eye-related morphological features, likely reflect adaptations to the pelagic world of reef fish larvae, either to degrees of darkness or differing wavelengths of light. Fortunately, these shapes tend to be consistent within species and can be used as characters to help identify larvae.
      larval eyes fish
    The primary variations are in eyeball shape, most often a narrowed vertical oval, but sometimes squared or another irregular shape. The oval sometimes can show a pronounced tilt, usually forward, but sometimes backward. The direction of the tilt is not always consistent within larval types, for example larval Evorthodus lyricus commonly show tilts both forward and backward (this is true to a lesser degree for larval parrotfishes, family Scaridae, as well as the wrasses of family Labridae). As a rule, the eyes of larval gobies become fully round at the end of the settlement transition.
    larval fish eye  
    In addition, there can be indentations in the iris, usually, but not always, dorsal and/or ventral. Many very early-stage larvae of all kinds of fishes show these indentations as part of the development of the eyeball, but in larval gobies these indentations can persist, sometimes through transition. Persistent indentations in various quadrants of the iris can be a consistent character for certain larval types. The photograph below left shows a persistent dorsal iris indentation in a 9.6 mm SL transitional larva of Ctenogobius saepepallens. The photograph below right shows a 5.5 mm SL Bathygobius curacao larva with persistent off-center-axis dorsal and ventral indentations of the iris despite being in transition.
    larval ctenogobius saepepallens larval fish eye
      ichthyoplankton eye
    Another occasional feature of the eyeball of larval gobies is the presence of an additional speckled membrane overlying the black surface of the upper iris. This feature is mostly consistent within larval types and can thus aid in identifications. In several larval goby types, this membrane is visibly lifted off from the eyeball. In some species the speckled membrane is only along the top quarter of the eyeball, while in others it extends further down, usually overlying the posterior half of the iris. At right, the oblong-shaped eyeball of a 7.2 mm SL larval Coryphopterus glaucofraenum shows the distinctly speckled membrane overlying the upper and rear of the iris.
    larval eye abnormality  
    A very common feature in the eyes of larval gobies is an extension of the shiny iris in the posterior-inferior quadrant. The extension appears to have a more flattened appearance than the rest of the iris. In some larval types this extension is quite prominent. Some rare individuals show clearly abnormal outgrowths of the eyeball in this same quadrant, perhaps a developmental anomaly related to whatever might be the function of this extension. The photograph at left shows a 6.9 mm SL larval Coryphopterus glaucofraenum with the abnormal outgrowth.
     
    A rare feature in some larval gobies is a bizarre outgrowth of tissue from the eyeball into the adjacent compartments of the head. Interestingly, in Microgobius signatus this can occur in several individuals in the same collection, suggesting that whatever is causing the anomaly may be an environmental effect. The photograph below shows the head of an 8.0 mm larval Microgobius signatus.
    abnormal larval development
    pelvic-fin morphology
      larval elacatinus saucrus
    Gobies are perhaps best known for their fused pelvic fins that act as a sucking disk to anchor them to the substrate. The degree of fusion of the pelvic fins and the overall shape of the disk are important characters in gobioid taxonomy, although the feature is certainly far more labile than taxonomists would desire. Unfortunately the degree of concordance between larvae and adults in pelvic-fin morphology is still an open question. In my collections, it is clear that the presence or absence of divided pelvic fins can differ between larval and adult stages, as in Coryphopterus personatus.
       
    larval gobulus pelvic fin  
    There are several basic states of pelvic-fin morphology in larval gobies. The pelvic fins on the right and left can be completely separate, with the base of the innermost fin ray clearly separated by a space from the base of the ray on the other side. This state is typical of most fishes (including the gobioid sleepers of the family Eleotridae), but is quite uncommon in gobies. The pelvic fins can be divided down to the base, or only partially-divided, leaving the proximal innermost fin rays still fused (as in larval Gobulus myersi, pictured at left). Alternatively, the pelvic fins can be completely fused along the length of the rays; this is the most
    common condition among the larval gobies. Lastly, within the completely-fused pelvic-fin group, there can be a frenum, or anterior connecting band, joining the outermost pelvic-fin spines on the two sides to form a cup-shaped fin. This cup can be flat and inconspicuous, as in the 9.9 mm SL larval Ctenogobius saepepallens at right, or an obvious large sucking disk as in the 7.7 mm SL larval Elacatinus saucrus pictured at the top of this section. fused pelvic fin goby

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